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Int J Mol Med 37(5):12211228 Shabbir A, Shahzad M, Ali A, Zia-ur-Rehman M (2014) Anti-arthritic activity of N-[(2, 4-dihydroxyphenyl) methylidene]-2-(3, 4-dimethyl-5, 5-dioxidopyrazolo [4, 3-c][1, 2] benzothiazin-1 (4H)-yl) acetohydrazide

Copper-Dependent Enzyme Activation GHK-Cu serves as a bioavailable copper delivery system, providing copper(II) ions to copper-dependent enzymes including superoxide dismutase (SOD), cytochrome c oxidase, and lysyl oxidase

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2 Biosynthesis of melanin and its regulation 2.1 Melanin synthesis pathway The specific process of melanin synthesis begins with the conversion of tyrosine into L-Dopa catalysed by tyrosinase, the central glycoprotease in the melanosome region and the only rate-limiting enzyme in this process (Kim et al., 2023)

DNA was isolated using the Qiagen Plasmid Mini kit or the Qiagen Plasmid Plus Midi kit